HTATIP (2OU2)
(Plasmid #33338)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q1 & Q2 2011 (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: Structural Genomics Consortium, Addgene plasmid 26094
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HTATIP
• Alt name: PDB: 2OU2
• Alt name: HIV-1 Tat interactive protein, 60kDa isoform 2
• Alt name: cPLA2, ESA1, HTATIP1, KAT5, PLIP, TIP, TIP60
• Species: H. sapiens (human)
• Insert Size (bp): 840
• Entrez Gene: KAT5 (a.k.a. ESA1, HTATIP, HTATIP1, NEDFASB, PLIP, TIP, TIP60, ZC2HC5, cPLA2)
• Promoter: T7
• Tag / Fusion Protein:
  • Hexahistidine tag with Thrombin cleavage (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 2OU2. http://www.thesgc.org/structures/2OU2/