pMSCV-Atoh1wt-IRES-RFP
(Plasmid #33334)

• Depositing Labs: Martine Roussel, Charles Sherr
• Publication: Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. Epub 2010 Jun 1.

Backbone

• Vector backbone: pMSCV-IRES-RFP
• Backbone manufacturer: Addgene plasmid #33337
• Backbone size w/o insert (bp): 6575
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Atoh1
• Species: M. musculus (mouse)
• Insert Size (bp): 1200
• Entrez Gene: Atoh1 (a.k.a. Hath1, MATH-1, Math1, bHLHa14)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLXSN 5'

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Addgene sequencing results determined that the BglII and XhoI sites are missing from the 3' end of the MCS and were likely destroyed during cloning.