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pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha
(Plasmid #33330)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33330 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Calcium/Calmodulin dependent protein kinase kinase alpha
  • Alt name
    CaMKKalpha
  • Alt name
    CaMKK1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1623
  • Mutation
    CaMKKbeta N-terminus (1-164) was fused to body for CaMKKalpha (121-505) using PCR based method; S3V, G96R, and P108L also present.
  • GenBank ID
    NP_113850
  • Entrez Gene
    Camkk1
  • Promoter SV40
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha was a gift from Anthony Means (Addgene plasmid # 33330 ; http://n2t.net/addgene:33330 ; RRID:Addgene_33330)

  • For your References section:

    Characterization of the CaMKKbeta-AMPK signaling complex. Green MF, Anderson KA, Means AR. Cell Signal. 2011 Dec;23(12):2005-12. Epub 2011 Jul 23. 10.1016/j.cellsig.2011.07.014 PubMed 21807092
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