pSG5-FLAG-CaMKKalpha FL WT
(Plasmid
#33327)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 33327 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSG5
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4100
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Modifications to backboneAdded blunt end cloning site which was lost during cloning.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCalcium/Calmodulin dependent protein kinase kinase alpha
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Alt nameCaMKKalpha
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Alt nameCaMKK1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1500
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GenBank IDNP_113850
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Entrez GeneCamkk1
- Promoter SV40
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5-FLAG-CaMKKalpha FL WT was a gift from Anthony Means (Addgene plasmid # 33327 ; http://n2t.net/addgene:33327 ; RRID:Addgene_33327) -
For your References section:
Characterization of the CaMKKbeta-AMPK signaling complex. Green MF, Anderson KA, Means AR. Cell Signal. 2011 Dec;23(12):2005-12. Epub 2011 Jul 23. 10.1016/j.cellsig.2011.07.014 PubMed 21807092