pSG5-FLAG-CaMKKbeta rat 1-460 D311A
(Plasmid #33325)

• Depositing Lab: Anthony Means
• Publication: Green et al Cell Signal. 2011 Dec;23(12):2005-12. Epub 2011 Jul 23.

Backbone

• Vector backbone: pSG5
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4100
• Modifications to backbone: Added blunt end cloning site which was lost during cloning.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Calcium/Calmodulin dependent protein kinase kinase beta
• Alt name: CaMKKbeta
• Alt name: CaMKK2
• Species: R. norvegicus (rat)
• Insert Size (bp): 1700
• Mutation: truncated at 460, D311A (S3V, G96R, and P108L also present)
• GenBank ID: NM_031338
• Entrez Gene: Camkk2
• Promoter: SV40
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (destroyed during cloning)
• 3′ cloning site: None (destroyed during cloning)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The truncation was made by placing a stop codon at 460, but sequence was not actually removed.

The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.