CaYin1 NES
(Plasmid #33318)

• Depositing Lab: Robert Campbell
• Publication: Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 2011 Nov 28.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5500
• Modifications to backbone: multiple cloning sites have been modified and it has the same reading frame as in pBAD hisB vector
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CaYin1 NES
• Alt name: tdtomato mAmetrine1.2 FRET calcium sensor
• Species: synthetic construct
• Insert Size (bp): 2652
• GenBank ID: JQ031556
• Promoter: CMV
• Tag / Fusion Protein:
  • NES sequence LALKLAGLDIGS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: T7_fwr_primer
• 3′ sequencing primer: BGH_rev_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry