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Addgene

p3HA-hMIB1
(Plasmid #33317)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33317 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCX-HA
  • Modifications to backbone
    pCX-HA, based on pcDNA3, but containing a modified CMV promoter originally obtained from vector RK5 (Pharmingen)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIB1
  • Species
    H. sapiens (human)
  • Entrez Gene
    MIB1 (a.k.a. DIP-1, DIP1, LVNC7, MIB, ZZANK2, ZZZ6)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer CMV forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3HA-hMIB1 was a gift from Vanessa Redecke (Addgene plasmid # 33317 ; http://n2t.net/addgene:33317 ; RRID:Addgene_33317)

  • For your References section:

    The E3 Ubiquitin Ligase Mind Bomb-2 (MIB2) Protein Controls B-cell CLL/Lymphoma 10 (BCL10)-dependent NF-{kappa}B Activation. Stempin CC, Chi L, Giraldo-Vela JP, High AA, Hacker H, Redecke V. J Biol Chem. 2011 Oct 28;286(43):37147-57. Epub 2011 Sep 6. 10.1074/jbc.M111.263384 PubMed 21896478
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