p3HA-hMIB1
(Plasmid #33317)

• Depositing Lab: Vanessa Redecke
• Publication: Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-57. Epub 2011 Sep 6.

Backbone

• Vector backbone: pCX-HA
• Modifications to backbone: pCX-HA, based on pcDNA3, but containing a modified CMV promoter originally obtained from vector RK5 (Pharmingen)
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MIB1
• Species: H. sapiens (human)
• Entrez Gene: MIB1 (a.k.a. DIP-1, DIP1, LVNC7, MIB, ZZANK2, ZZZ6)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xHA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: CMV forward

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry