pGST-mBCL10
(Plasmid
#33316)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 33316 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T-3
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBcl10
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SpeciesM. musculus (mouse)
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Entrez GeneBcl10 (a.k.a. AI132454, BCL-1, BCL-10, C81403, CARMEN, CIPER, CLAP, ME10, cE10)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer pGEX Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGST-mBCL10 was a gift from Vanessa Redecke (Addgene plasmid # 33316 ; http://n2t.net/addgene:33316 ; RRID:Addgene_33316) -
For your References section:
The E3 Ubiquitin Ligase Mind Bomb-2 (MIB2) Protein Controls B-cell CLL/Lymphoma 10 (BCL10)-dependent NF-{kappa}B Activation. Stempin CC, Chi L, Giraldo-Vela JP, High AA, Hacker H, Redecke V. J Biol Chem. 2011 Oct 28;286(43):37147-57. Epub 2011 Sep 6. 10.1074/jbc.M111.263384 PubMed 21896478
Map uploaded by the depositor.
