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Addgene

p3F-Strep-hMIB2
(Plasmid #33313)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-FLAG-Strep
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIB2
  • Species
    H. sapiens (human)
  • Entrez Gene
    MIB2 (a.k.a. ZZANK1, ZZZ5)
  • Promoter EF1a
  • Tags / Fusion Proteins
    • 3xFlag (N terminal on backbone)
    • StrepOne (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer EF1a forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3F-Strep-hMIB2 was a gift from Vanessa Redecke (Addgene plasmid # 33313 ; http://n2t.net/addgene:33313 ; RRID:Addgene_33313)

  • For your References section:

    The E3 Ubiquitin Ligase Mind Bomb-2 (MIB2) Protein Controls B-cell CLL/Lymphoma 10 (BCL10)-dependent NF-{kappa}B Activation. Stempin CC, Chi L, Giraldo-Vela JP, High AA, Hacker H, Redecke V. J Biol Chem. 2011 Oct 28;286(43):37147-57. Epub 2011 Sep 6. 10.1074/jbc.M111.263384 PubMed 21896478
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