mSIRT3-L
(Plasmid #33309)

• Depositing Lab: Michael Sack
• Publication: Bao et al J Cell Biochem. 2010 May;110(1):238-47.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sirt3
• Species: M. musculus (mouse)
• Insert Size (bp): 960
• Entrez Gene: Sirt3 (a.k.a. 2310003L23Rik, Sir2l3)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3XFlag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-Rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry