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Addgene

Ubc.Luc.IRES.Puro
(Plasmid #33307)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33307 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    cFUGW
  • Backbone manufacturer
    David Baltimore
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Firefly Luciferase
  • Alt name
    fLuc
  • Species
    firefly
  • Insert Size (bp)
    1652
  • Promoter Ubc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer gaactatgcgctcggggttgg
  • 3′ sequencing primer agaagacagggccaggtttcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ubc.Luc.IRES.Puro was a gift from Linzhao Cheng (Addgene plasmid # 33307 ; http://n2t.net/addgene:33307 ; RRID:Addgene_33307)

  • For your References section:

    Molecular imaging and stem cell research. Jang YY, Ye Z, Cheng L. Mol Imaging. 2011 Apr;10(2):111-22. PubMed 21439256
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