pT7-L3-S3T1L
(Plasmid
#33305)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 33305 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3E5EGFP
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Backbone manufacturermade by Yoshihiro Kawaoka
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameL3
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GenBank ID
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Entrez GeneMoV1sL3gp1 (a.k.a. MoV1sL3gp1)
- Promoter T7
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer ovL3T1L-F (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameS3
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GenBank ID
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Entrez GeneMoV1sS3gp1 (a.k.a. MoV1sS3gp1)
- Promoter T7
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7-L3-S3T1L was a gift from Terence Dermody (Addgene plasmid # 33305 ; http://n2t.net/addgene:33305 ; RRID:Addgene_33305) -
For your References section:
An improved reverse genetics system for mammalian orthoreoviruses. Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS. Virology. 2010 Mar 15;398(2):194-200. Epub 2009 Dec 29. 10.1016/j.virol.2009.11.037 PubMed 20042210