pT7-M2-S2-S3-S4T3D
(Plasmid #33302)

• Depositing Lab: Terence Dermody
• Publication: Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Epub 2009 Dec 29.

Backbone

• Vector backbone: p3E5EGFP
• Backbone manufacturer: made by Yoshihiro Kawaoka
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: S2
• Species: Orthoreovirus T3D
• Entrez Gene: S2 (a.k.a. MoV-3mpc04sS2_gp1)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: ovS2T3D-F

Gene/Insert 2

• Gene/Insert name: S3
• Species: Orthoreovirus T3D
• Entrez Gene: S3 (a.k.a. MoV-3mpc04sS3_gp1)
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: none

Gene/Insert 3

• Gene/Insert name: S4
• Species: Orthoreovirus T3D
• Entrez Gene: S4 (a.k.a. MoV-3mpc04sS4_gp1)
• Promoter: T7

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: none

Gene/Insert 4

• Gene/Insert name: M2
• Species: Orthoreovirus T3D
• Entrez Gene: M2 (a.k.a. MoV-3mpc04sM2_gp1)
• Promoter: T7

Cloning Information for Gene/Insert 4

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: ovM2T3D-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry