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Addgene

pT7-L1-S1T3D
(Plasmid #33299)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33299 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p3E5EGFP
  • Backbone manufacturer
    made by Yoshihiro Kawaoka
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    L1
  • Species
    Orthoreovirus T3D
  • Entrez Gene
    L1 (a.k.a. MoV3sL1gp1)
  • Entrez Gene
    L1 (a.k.a. MoV-3mpc04sL1_gp1)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer ovL1T3D-F
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    S1
  • Species
    Orthoreovirus T3D
  • Entrez Gene
    S1 (a.k.a. MoV-3mpc04sS1_gp1)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer none
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-L1-S1T3D was a gift from Terence Dermody (Addgene plasmid # 33299)

  • For your References section:

    An improved reverse genetics system for mammalian orthoreoviruses. Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS. Virology. 2010 Mar 15;398(2):194-200. Epub 2009 Dec 29. 10.1016/j.virol.2009.11.037 PubMed 20042210
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