pUNos_C1
(Plasmid
#33297)
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Purpose(Empty Backbone)
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 33297 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC18 and pAHC25
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Backbone manufacturerATCC(pUC18) and Christensen and Quail(pAHC25)
- Backbone size (bp) 4930
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Modifications to backbonepUC18 was digested by HindIII and EcoRI, then DNA fragment containing maize ubiquitin promoter, E. coli uidA gene(GUS) and NOS terminator which is obtained from pAHC25 was inserted. GUS region was cut out by using SmaI and SacI, and Gateway cassette C1 which is obtained from pMDC32 was inserted there. SmaI site was destroyed by this procedure.
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Vector typePlant expression
- Promoter maize ubiquitin (Ubi-1)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberLow Copy
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUNos_C1 was a gift from Jane Glazebrook & Fumi Katagiri (Addgene plasmid # 33297 ; http://n2t.net/addgene:33297 ; RRID:Addgene_33297)
Map uploaded by the depositor.
