pBacT7-S1T1L
(Plasmid
#33292)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 33292 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBacPAK8
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameS1
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SpeciesOrthoreovirus T1L
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GenBank ID
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Entrez GeneMoV1sS1gp2 (a.k.a. MoV1sS1gp2)
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Entrez GeneMoV1sS1gp1 (a.k.a. MoV1sS1gp1)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBacT7-S1T1L was a gift from Terence Dermody (Addgene plasmid # 33292 ; http://n2t.net/addgene:33292 ; RRID:Addgene_33292) -
For your References section:
A plasmid-based reverse genetics system for animal double-stranded RNA viruses. Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, Wilson GJ, Chappell JD, Dermody TS. Cell Host Microbe. 2007 Apr 19;1(2):147-57. 10.1016/j.chom.2007.03.003 PubMed 18005692
