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Addgene

pT7-L3T3D
(Plasmid #33278)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33278 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3E5EGFP
  • Backbone manufacturer
    made by Yoshihiro Kawaoka
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    L3
  • Species
    Orthoreovirus T3D
  • GenBank ID
    EF494437
  • Entrez Gene
    L3 (a.k.a. MoV-3mpc04sL3_gp1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (unknown if destroyed)
  • 3′ cloning site RsrII (unknown if destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-L3T3D was a gift from Terence Dermody (Addgene plasmid # 33278 ; http://n2t.net/addgene:33278 ; RRID:Addgene_33278)

  • For your References section:

    A plasmid-based reverse genetics system for animal double-stranded RNA viruses. Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, Wilson GJ, Chappell JD, Dermody TS. Cell Host Microbe. 2007 Apr 19;1(2):147-57. 10.1016/j.chom.2007.03.003 PubMed 18005692
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