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pMXs-mGLIS1
(Plasmid #33273)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33273 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMXs
  • Backbone manufacturer
    Dr. Toshio Kitamura
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLIS1, Gateway casette A
  • Alt name
    RP23-399H3.1, Gli5, Gli6, GliH1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2438
  • GenBank ID
    230587
  • Entrez Gene
    Glis1 (a.k.a. Gli5, Gli6, GliH1)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway cassette is from Invitrogen Corporation.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-mGLIS1 was a gift from Shinya Yamanaka (Addgene plasmid # 33273 ; http://n2t.net/addgene:33273 ; RRID:Addgene_33273)

  • For your References section:

    Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Maekawa M, Yamaguchi K, Nakamura T, Shibukawa R, Kodanaka I, Ichisaka T, Kawamura Y, Mochizuki H, Goshima N, Yamanaka S. Nature. 2011 . 474(7350):225-9. 10.1038/nature10106 PubMed 21654807
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