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pCasper_Pdf-LexA::VP16
(Plasmid #33259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCasPeR3
  • Modifications to backbone
    The 2.4-kb Pdf promoter (Stoleru et al., 2004) was cloned into pCasPeR3 between BamHI and BglII sites.
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LexA::VP16-SV40 3'UTR
  • Species
    E. coli
  • Entrez Gene
    lexA (a.k.a. b4043, ECK4035, exrA, spr, tsl, umuA)
  • Promoter Pdf

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer SV40 polyA reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A XbaI site was added to the 3' end of LexAVP16-SV40 3'UTR by PCR from pBS-LexAVP16 plasmid (Lai and Lee, 2006).
The PCR fragment was then subcloned into the XbaI site of the pBS vector.
LexAVP16-SV40 was then released by NotI digestion and inserted into the NotI site in pCaspeR3, downstream of Pdf promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCasper_Pdf-LexA::VP16 was a gift from Michael Rosbash (Addgene plasmid # 33259 ; http://n2t.net/addgene:33259 ; RRID:Addgene_33259)

  • For your References section:

    Light-arousal and circadian photoreception circuits intersect at the large PDF cells of the Drosophila brain. Shang Y, Griffith LC, Rosbash M. Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19587-94. Epub 2008 Dec 5. 10.1073/pnas.0809577105 PubMed 19060186
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