pBabe puro-HA-PIK3CA(H1047R;G320E)
(Plasmid #33254)

• Depositing Lab: Thomas Roberts
• Publication: Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):E699-708. Epub 2011 Aug 29.

Backbone

• Vector backbone: pBabe puro
• Backbone size w/o insert (bp): 5168
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PIK3CA
• Species: H. sapiens (human)
• Insert Size (bp): 3200
• Mutation: changed histidine 1047 to arginine and glycine 320 to glutamic acid
• Entrez Gene: PIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)
• Promoter: SV40
• Tag / Fusion Protein:
  • Hemagglutinin (HA) (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CTTTATCCAGCCCTCAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: from JJ Zhao, DFCI, Boston MA.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note from the depositing lab: "This plasmid contains an additional mutation, I143V, that is not thought to alter function of the protein"