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pBabe puro-HA-PIK3CA(H1047R;I848V)
(Plasmid #33253)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33253 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe puro
  • Backbone size w/o insert (bp) 5168
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIK3CA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3200
  • Mutation
    changed histidine 1047 to arginine and isoleucine 848 to valine
  • Entrez Gene
    PIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)
  • Promoter SV40
  • Tag / Fusion Protein
    • Hemagglutinin (HA) (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    from JJ Zhao, DFCI, Boston MA.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note from the depositing lab: "This plasmid contains an additional mutation, I143V, that is not thought to alter function of the protein"

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe puro-HA-PIK3CA(H1047R;I848V) was a gift from Thomas Roberts (Addgene plasmid # 33253 ; http://n2t.net/addgene:33253 ; RRID:Addgene_33253)

  • For your References section:

    PI3K-targeted therapy can be evaded by gene amplification along the MYC-eukaryotic translation initiation factor 4E (eIF4E) axis. Ilic N, Utermark T, Widlund HR, Roberts TM. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):E699-708. Epub 2011 Aug 29. 10.1073/pnas.1108237108 PubMed 21876152
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