pBabe puro-eIF4E
(Plasmid #33252)

• Depositing Lab: Thomas Roberts
• Publication: Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):E699-708. Epub 2011 Aug 29.

Backbone

• Vector backbone: pBabe puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eIF4E
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: unmodified
• Entrez Gene: EIF4E (a.k.a. AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E)
• Promoter: SV40
• Tag / Fusion Protein:
  • untagged

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (unknown if destroyed)
• 3′ cloning site: SnaBI (unknown if destroyed)
• 5′ sequencing primer: CTTTATCCAGCCCTCAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: from Dong-Er Zhang, University of California at San Diego, La Jolla, CA from the pHA-eIF4E construct Addgene plasmid 17343
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry