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pcDNA3.1/FGFR2-S252W(Apert) myc
(Plasmid #33249)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33249 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FGFR2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3070
  • Mutation
    Mutation S252W in FGFR2 found in Apert syndrome. cDNA is a 3-Ig domain (IIIC) isoform of FGFR2. KpnI site introduced at stop codon to be inframe with myc-his tag from pcDNA vector
  • GenBank ID
    M86441
  • Entrez Gene
    Fgfr2 (a.k.a. Bek, Fgfr-2, Fgfr-7, Fgfr2b, Fgfr7, KGFR, KGFRTr, svs)
  • Promoter CMV
  • Tags / Fusion Proteins
    • His (C terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/FGFR2-S252W(Apert) myc was a gift from Claudio Basilico & Alka Mansukhani (Addgene plasmid # 33249 ; http://n2t.net/addgene:33249 ; RRID:Addgene_33249)

  • For your References section:

    Signaling by fibroblast growth factors (FGF) and fibroblast growth factor receptor 2 (FGFR2)-activating mutations blocks mineralization and induces apoptosis in osteoblasts. Mansukhani A, Bellosta P, Sahni M, Basilico C. J Cell Biol. 2000 Jun 12. 149(6):1297-308. 10.1083/jcb.149.6.1297 PubMed 10851026
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