pcDNA3.1/FGFR2myc
(Plasmid #33248)

• Depositing Labs: Claudio Basilico, Alka Mansukhani
• Publication: Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-308.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FGFR2
• Species: M. musculus (mouse)
• Insert Size (bp): 3070
• Mutation: KpnI site introduced at stop codon of FGFR2 to be inframe with myc-his from pcDNA3 backbone
• GenBank ID: M86441
• Entrez Gene: Fgfr2 (a.k.a. Bek, Fgfr-2, Fgfr-7, Fgfr2b, Fgfr7, KGFR, KGFRTr, svs)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Myc (C terminal on insert)
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry