pcDNA1 3XFlag ATXN1[15Q]-delAXH
(Plasmid
#33241)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 33241 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA1.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATXN1[2Q]-delAXH
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SpeciesH. sapiens (human)
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Entrez GeneATXN1 (a.k.a. ATX1, D6S504E, SCA1)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xflag
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA1 3XFlag ATXN1[15Q]-delAXH was a gift from Huda Zoghbi (Addgene plasmid # 33241 ; http://n2t.net/addgene:33241 ; RRID:Addgene_33241) -
For your References section:
ATAXIN-1 interacts with the repressor Capicua in its native complex to cause SCA1 neuropathology. Lam YC, Bowman AB, Jafar-Nejad P, Lim J, Richman R, Fryer JD, Hyun ED, Duvick LA, Orr HT, Botas J, Zoghbi HY. Cell. 2006 Dec 29;127(7):1335-47. 10.1016/j.cell.2006.11.038 PubMed 17190598