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Addgene

pcDNA1 3XFlag ATXN1[15Q]-delAXH
(Plasmid #33241)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33241 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA1.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATXN1[2Q]-delAXH
  • Species
    H. sapiens (human)
  • Entrez Gene
    ATXN1 (a.k.a. ATX1, D6S504E, SCA1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xflag

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA1 3XFlag ATXN1[15Q]-delAXH was a gift from Huda Zoghbi (Addgene plasmid # 33241 ; http://n2t.net/addgene:33241 ; RRID:Addgene_33241)
  • For your References section:

    ATAXIN-1 interacts with the repressor Capicua in its native complex to cause SCA1 neuropathology. Lam YC, Bowman AB, Jafar-Nejad P, Lim J, Richman R, Fryer JD, Hyun ED, Duvick LA, Orr HT, Botas J, Zoghbi HY. Cell. 2006 Dec 29;127(7):1335-47. 10.1016/j.cell.2006.11.038 PubMed 17190598