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Addgene

pEGFP-ATXN1-30Q
(Plasmid #33238)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ataxin-1
  • Alt name
    ATXN1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2448
  • Mutation
    insert contains a stretch of 32 amino acids (30 Q interrupted by 2 H) and no stop codon
  • GenBank ID
    NM_000332
  • Entrez Gene
    ATXN1 (a.k.a. ATX1, D6S504E, SCA1)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-ATXN1-30Q was a gift from Huda Zoghbi (Addgene plasmid # 33238 ; http://n2t.net/addgene:33238 ; RRID:Addgene_33238)

  • For your References section:

    Chaperone suppression of aggregation and altered subcellular proteasome localization imply protein misfolding in SCA1. Cummings CJ, Mancini MA, Antalffy B, DeFranco DB, Orr HT, Zoghbi HY. Nat Genet. 1998 Jun;19(2):148-54. 10.1038/502 PubMed 9620770
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