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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 33236 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA1.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtaxin-1
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Alt nameATXN1
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SpeciesH. sapiens (human)
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Insert Size (bp)2448
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Mutationinsert contains a stretch of 29 amino acids (27 Q interrupted by 2 H) and no stop codon
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GenBank IDNM_000332
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Entrez GeneATXN1 (a.k.a. ATX1, D6S504E, SCA1)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA1 Flag ATXN1[30Q] was a gift from Huda Zoghbi (Addgene plasmid # 33236 ; http://n2t.net/addgene:33236 ; RRID:Addgene_33236) -
For your References section:
Ataxin-1 with an expanded glutamine tract alters nuclear matrix-associated structures. Skinner PJ, Koshy BT, Cummings CJ, Klement IA, Helin K, Servadio A, Zoghbi HY, Orr HT. Nature. 1997 Oct 30;389(6654):971-4. 10.1038/40153 PubMed 9353120