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Addgene

Gal-Rad51
(Plasmid #33230)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33230 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYES2
  • Backbone size w/o insert (bp) 5857
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rad51
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RAD51 (a.k.a. YER095W, MUT5)
  • Promoter Gal1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site SphI (unknown if destroyed)
  • 5′ sequencing primer Gal1_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gal-Rad51 was a gift from Hannah Klein (Addgene plasmid # 33230 ; http://n2t.net/addgene:33230 ; RRID:Addgene_33230)

  • For your References section:

    Swi2/Snf2-related translocases prevent accumulation of toxic Rad51 complexes during mitotic growth. Shah PP, Zheng X, Epshtein A, Carey JN, Bishop DK, Klein HL. Mol Cell. 2010 Sep 24;39(6):862-72. 10.1016/j.molcel.2010.08.028 PubMed 20864034
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