pDMG1p
(Plasmid
#33226)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 33226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIS1
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Backbone manufacturerAddgene (#12179)
- Backbone size w/o insert (bp) 4085
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecog-1 3'UTR
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SpeciesC. elegans (nematode)
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Mutation1st, 2nd site GU6,8
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GenBank IDNM_001027093
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Entrez Genecog-1 (a.k.a. R03C1.3)
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Tag
/ Fusion Protein
- Luciferase (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EagI (not destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer EBV Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDMG1p was a gift from David Bartel (Addgene plasmid # 33226 ; http://n2t.net/addgene:33226 ; RRID:Addgene_33226) -
For your References section:
Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs. Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP. Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 10.1038/nsmb.2115. 10.1038/nsmb.2115 PubMed 21909094