pAG252
(Plasmid
#33206)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 33206 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIS2
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Backbone manufacturerAddgene plasmid #12177
- Backbone size w/o insert (bp) 3745
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMAP4K4 3'UTR
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SpeciesH. sapiens (human)
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GenBank IDNM_145686.2
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Entrez GeneMAP4K4 (a.k.a. FLH21957, HEL-S-31, HGK, MEKKK4, NIK)
- Promoter SV40 early enhancer/promoter
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Tag
/ Fusion Protein
- Luciferase (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer Rluc-F
- 3′ sequencing primer EBV Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG252 was a gift from David Bartel (Addgene plasmid # 33206 ; http://n2t.net/addgene:33206 ; RRID:Addgene_33206) -
For your References section:
Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs. Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP. Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 10.1038/nsmb.2115. 10.1038/nsmb.2115 PubMed 21909094