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Addgene

pCS250
(Plasmid #33205)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIS2
  • Backbone manufacturer
    Addgene plasmid #12177
  • Backbone size w/o insert (bp) 3745
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NEK6 3'UTR
  • Species
    H. sapiens (human)
  • Mutation
    miR-CGCG sites: aaCgCgaa, aaCgCgaa
  • GenBank ID
    NM_001145001.2
  • Entrez Gene
    NEK6 (a.k.a. SID6-1512)
  • Promoter SV40 early enhancer/promoter
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer Rluc-F
  • 3′ sequencing primer EBV Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results found a single nucleotide mismatch at bp#786 when compared to the sequence from the depositing laboratory. The depositing scientist states that the mismatch is not a concern for the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS250 was a gift from David Bartel (Addgene plasmid # 33205 ; http://n2t.net/addgene:33205 ; RRID:Addgene_33205)

  • For your References section:

    Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs. Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP. Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 10.1038/nsmb.2115. 10.1038/nsmb.2115 PubMed 21909094
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