pAC-Per
(Plasmid #33157)

• Depositing Lab: Michael Rosbash
• Publication: Nawathean et al Mol Cell. 2004 Jan 30;13(2):213-23.

Backbone

• Vector backbone: pAc5.1/V5-His B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Insect Expression, Retroviral
• Selectable markers: Hygromycin, Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Per
• Species: D. melanogaster (fly)
• Entrez Gene: per (a.k.a. Dmel_CG2647, CG2647, Clk, Dmel\CG2647, EG:155E2.4, PER, Per, Per-2, clk-6, dPER, dPer, dmper, dper, mel_per)
• Promoter: Actin 5C
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • 6xHIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: AC5

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry