Skip to main content
Addgene

pAC-Cyc
(Plasmid #33156)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33156 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAc5.1/V5-His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Insect Expression, Retroviral
  • Selectable markers
    Hygromycin, Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cyc
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    cyc (a.k.a. Dmel_CG8727, BMAL1, Bmal1, CG8727, CYC, CYCLE, Cyc, Cycle, Dmel\CG8727, MOP3, Mop3, TGO_CYC, bHLHe7, bMAL1, dBMAL, dBMAL1, dBma1, dBmal, dCYC, dbmal, dbmal1)
  • Promoter Actin 5C
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6xHIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer AC5
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-Cyc was a gift from Michael Rosbash (Addgene plasmid # 33156 ; http://n2t.net/addgene:33156 ; RRID:Addgene_33156)

  • For your References section:

    Circadian transcription contributes to core period determination in Drosophila. Kadener S, Menet JS, Schoer R, Rosbash M. PLoS Biol. 2008 May 20;6(5):e119. 10.1371/journal.pbio.0060119 PubMed 18494558
Related items:
Commonly requested with: