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pAC-cwo
(Plasmid #33155)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33155 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1/V5-His B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Insect Expression, Retroviral
  • Selectable markers
    Hygromycin, Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cwo
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    cwo (a.k.a. Dmel_CG17100, AAF24476.1, CG17100, CWO, Dmel\CG17100, GM05287, bHLHe49)
  • Promoter Actin 5C
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6xHIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer AC5
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AKA: pAC-Sti-V5

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-cwo was a gift from Michael Rosbash (Addgene plasmid # 33155 ; http://n2t.net/addgene:33155 ; RRID:Addgene_33155)

  • For your References section:

    Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component. Kadener S, Stoleru D, McDonald M, Nawathean P, Rosbash M. Genes Dev. 2007 Jul 1;21(13):1675-86. Epub 2007 Jun 19. 10.1101/gad.1552607 PubMed 17578907
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