pAC-dCLK
(Plasmid #33153)

• Depositing Lab: Michael Rosbash
• Publication: McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

Backbone

• Vector backbone: pAc5.1/V5-His B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Insect Expression, Retroviral
• Selectable markers: Hygromycin, Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CLK
• Species: D. melanogaster (fly)
• Entrez Gene: Clk (a.k.a. Dmel_CG7391, CG7391, CLK, CLOCK, Dmel\CG7391, Jerk, Jrk, PAS1, bHLHe10, clk, clock, dCLK, dCLK/JRK, dCLOCK, dClck, dClk, dClock, dclk, jrk)
• Promoter: Actin 5C
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • 6xHIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AC5

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry