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Addgene

pRS315-nls-T7-RNAP
(Plasmid #33152)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33152 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS315 GPD
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    TG1
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    T7 RNAP
  • Alt name
    T7 RNA Polymerase
  • Species
    E. coli [BL21(DE3)]
  • Insert Size (bp)
    3000
  • Promoter GPD
  • Tag / Fusion Protein
    • SV40 NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GPDpro-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS315-nls-T7-RNAP was a gift from Michael Rosbash (Addgene plasmid # 33152)

  • For your References section:

    T7 RNA polymerase-directed transcripts are processed in yeast and link 3' end formation to mRNA nuclear export. Dower K, Rosbash M. RNA. 2002 May;8(5):686-97. 10.1017/S1355838202024068 PubMed 12022234
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