-
Purposegenomic GFP tagging in yeast
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 33141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFA6a
-
Selectable markersZeocin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert namebleMX6
Gene/Insert 2
-
Gene/Insert nameGFP
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is a mutation in the Bleo gene, but it does not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFA6a-GFP-bleMX6 was a gift from Eishi Noguchi (Addgene plasmid # 33141 ; http://n2t.net/addgene:33141 ; RRID:Addgene_33141) -
For your References section:
New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe. Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E. Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100. 10.2144/000114100 PubMed 24215641