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Addgene

pFA6a-GFP-bleMX6
(Plasmid #33141)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33141 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFA6a
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    bleMX6

Gene/Insert 2

  • Gene/Insert name
    GFP

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a mutation in the Bleo gene, but it does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-GFP-bleMX6 was a gift from Eishi Noguchi (Addgene plasmid # 33141 ; http://n2t.net/addgene:33141 ; RRID:Addgene_33141)
  • For your References section:

    New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe. Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E. Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100. 10.2144/000114100 PubMed 24215641