pFA6a-his3MX6
(Plasmid
#33139)
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Purposegene deletion in S. pombe
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 33139 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehis3MX6
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SpeciesS. pombe (fission yeast)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note this is NOT the same plasmid described in "Heterologous His3 marker and GFP reporter modules for PCR targeting in S.
cerevisiae" by Wach et al. (1997).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-his3MX6 was a gift from Eishi Noguchi (Addgene plasmid # 33139 ; http://n2t.net/addgene:33139 ; RRID:Addgene_33139) -
For your References section:
New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe. Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E. Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100. 10.2144/000114100 PubMed 24215641
Map uploaded by the depositor.
