pAC-luc-CLK
(Plasmid #33132)

• Depositing Lab: Michael Rosbash
• Publication: Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20.

Backbone

• Vector backbone: pAc5.1/V5-His B
• Backbone manufacturer: Invitrogen
• Modifications to backbone: pAc-Luciferase was constructed by ligation of the PCR product containing the Renilla Luciferase gene and an artificial poly-linker sequence into the parent vector.
• Vector type: Insect Expression, Luciferase
• Selectable markers: Hygromycin, Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CLK 3' UTR
• Species: D. melanogaster (fly)
• Entrez Gene: Clk (a.k.a. Dmel_CG7391, CG7391, CLK, CLOCK, Dmel\CG7391, Jerk, Jrk, PAS1, bHLHe10, clk, clock, dCLK, dCLK/JRK, dCLOCK, dClck, dClk, dClock, dclk, jrk)
• Promoter: Actin 5C
• Tag / Fusion Protein:
  • Firefly Luciferase (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: AC5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pAc-Luc-clk was constructed by amplifying the genomic region containing the clk 3′ UTR plus 300 bases downstream from the annotated end of the mRNA and ligating the PCR product into pAc-Luciferase.