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pRS314-T7-GFP
(Plasmid #33130)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33130 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS314
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 4783
  • Modifications to backbone
    NotI - T7 promoter - TDH3 5'UTR - BamHI - GFP - SalI - TDH3 3' UTR and polyadenylation signal - EcoRI
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    TG1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP with TDH3 5' and 3' UTR
  • Entrez Gene
    gfp (a.k.a. pCmGFP_001)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7 forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T7 promoter driven GFP with the TDH3 5' and 3' flanking sequences, and the TDH3 polyadenylation signal all cloned into pRS314 using NotI and EcoRI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS314-T7-GFP was a gift from Michael Rosbash (Addgene plasmid # 33130 ; http://n2t.net/addgene:33130 ; RRID:Addgene_33130)

  • For your References section:

    T7 RNA polymerase-directed transcripts are processed in yeast and link 3' end formation to mRNA nuclear export. Dower K, Rosbash M. RNA. 2002 May;8(5):686-97. 10.1017/S1355838202024068 PubMed 12022234
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