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Addgene

VCDstubΔ741-752-GFP
(Plasmid #33123)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33123 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pcdh-γA3
  • Alt name
    protocadherin γA3
  • Species
    M. musculus (mouse)
  • Mutation
    Contains a signal sequence fused to a very small extracellular domain fragment and no constant domain and deletion of aa 741-752
  • Entrez Gene
    Pcdhga3
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VCDstubΔ741-752-GFP was a gift from Greg Phillips (Addgene plasmid # 33123 ; http://n2t.net/addgene:33123 ; RRID:Addgene_33123)

  • For your References section:

    A variable cytoplasmic domain segment is necessary for gamma-protocadherin trafficking and tubulation in the endosome/lysosome pathway. O'Leary R, Reilly J, Hanson HH, Kang S, Lou N, Phillips GR. Mol Biol Cell. 2011 Sep 14. 10.1091/mbc.E11-04-0283 PubMed 21917590
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