VCDstub-GFP
(Plasmid #33122)

• Depositing Lab: Greg Phillips
• Publication: O'Leary et al Mol Biol Cell. 2011 Sep 14.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pcdh-γA3
• Alt name: protocadherin γA3
• Species: M. musculus (mouse)
• Mutation: Contains a signal sequence fused to a very small extracellular domain fragment and no constant domain
• Entrez Gene: Pcdhga3
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry