pEGFP-hG9a-ΔSET
(Plasmid #33026)

• Depositing Lab: Martin Walsh
• Publication: Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11257-62. Epub 2004 Jul 21.

Backbone

• Vector backbone: pEGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 470
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hG9a
• Species: H. sapiens (human)
• Insert Size (bp): 2274
• Mutation: Deletion of the SET domain; contains aa 1-758
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry