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Addgene

pEGFP-hG9a
(Plasmid #33025)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33025 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hG9a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3003
  • GenBank ID
    AAD21812
  • Entrez Gene
    EHMT2 (a.k.a. BAT8, C6orf30, G9A, GAT8, KMT1C, NG36)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-hG9a was a gift from Martin Walsh (Addgene plasmid # 33025 ; http://n2t.net/addgene:33025 ; RRID:Addgene_33025)

  • For your References section:

    CCAAT displacement protein/cut homolog recruits G9a histone lysine methyltransferase to repress transcription. Nishio H, Walsh MJ. Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11257-62. Epub 2004 Jul 21. 10.1073/pnas.0401343101 PubMed 15269344
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