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pGL2-p21 promoter-Luc
(Plasmid #33021)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33021 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p21 promoter
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
  • Promoter p21
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer Luc_N_Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-p21 promoter-Luc was a gift from Martin Walsh (Addgene plasmid # 33021 ; http://n2t.net/addgene:33021 ; RRID:Addgene_33021)

  • For your References section:

    CCAAT displacement protein/cut homolog recruits G9a histone lysine methyltransferase to repress transcription. Nishio H, Walsh MJ. Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11257-62. Epub 2004 Jul 21. 10.1073/pnas.0401343101 PubMed 15269344
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