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Addgene

pCS2-bNWASP
(Plasmid #33019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone manufacturer
    RZPD
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-WASP
  • Species
    B. taurus (bovine)
  • Entrez Gene
    WASL (a.k.a. BOS_4408)
  • Promoter simian CMV IE94

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-bNWASP was a gift from Marc Kirschner (Addgene plasmid # 33019 ; http://n2t.net/addgene:33019 ; RRID:Addgene_33019)

  • For your References section:

    Self-assembly of filopodia-like structures on supported lipid bilayers. Lee K, Gallop JL, Rambani K, Kirschner MW. Science. 2010 Sep 10;329(5997):1341-5. 10.1126/science.1191710 PubMed 20829485
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