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Addgene

pCS2+HA-hSeparase
(Plasmid #33018)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone manufacturer
    RZPD
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Separase
  • Species
    H. sapiens (human)
  • Entrez Gene
    ESPL1 (a.k.a. ESP1, SEPA)
  • Promoter simian CMV IE94
  • Tag / Fusion Protein
    • 3xHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+HA-hSeparase was a gift from Marc Kirschner (Addgene plasmid # 33018 ; http://n2t.net/addgene:33018 ; RRID:Addgene_33018)

  • For your References section:

    Dual inhibition of sister chromatid separation at metaphase. Stemmann O, Zou H, Gerber SA, Gygi SP, Kirschner MW. Cell. 2001 Dec 14;107(6):715-26. 10.1016/S0092-8674(01)00603-1 PubMed 11747808
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