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Addgene

XActB-pSP64T
(Plasmid #32994)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32994 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64T
  • Backbone size w/o insert (bp) 3200

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    activin B
  • Alt name
    bB-64T
  • Alt name
    XActB
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1200

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linearize with EcoRI and transcribe with SP6 for sense XActivin bB mRNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XActB-pSP64T was a gift from Sergei Sokol (Addgene plasmid # 32994 ; http://n2t.net/addgene:32994 ; RRID:Addgene_32994)

  • For your References section:

    Injected Wnt RNA induces a complete body axis in Xenopus embryos. Sokol S, Christian JL, Moon RT, Melton DA. Cell. 1991 Nov 15;67(4):741-52. 10.1016/0092-8674(91)90069-B PubMed 1834344
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