ECD8-pCT7
(Plasmid
#32993)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32993 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCT7
- Backbone size w/o insert (bp) 5700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEcd8
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Alt nameXfz8
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SpeciesX. laevis (frog)
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MutationContains only the first 230 amino acids of Xfz8
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Entrez Genefzd8.L (a.k.a. XELAEV_18030715mg, Xfz8, frizzled8, fz8, fzd8, fzd8-a, fzd8-b)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV-Fwd
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ECD8-pCT7 was a gift from Sergei Sokol (Addgene plasmid # 32993 ; http://n2t.net/addgene:32993 ; RRID:Addgene_32993) -
For your References section:
Axis determination by inhibition of Wnt signaling in Xenopus. Itoh K, Sokol SY. Genes Dev. 1999 Sep 1;13(17):2328-36. 10.1101/gad.13.17.2328 PubMed 10485853
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/01/53/a0b75a4c-016d-11e1-9dc1-003048dd6500.pdf.940x940_q85_autocrop.png)