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Addgene

ECD8-pCT7
(Plasmid #32993)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32993 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCT7
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ecd8
  • Alt name
    Xfz8
  • Species
    X. laevis (frog)
  • Mutation
    Contains only the first 230 amino acids of Xfz8
  • Entrez Gene
    fzd8.L (a.k.a. XELAEV_18030715mg, Xfz8, frizzled8, fz8, fzd8, fzd8-a, fzd8-b)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ECD8-pCT7 was a gift from Sergei Sokol (Addgene plasmid # 32993 ; http://n2t.net/addgene:32993 ; RRID:Addgene_32993)

  • For your References section:

    Axis determination by inhibition of Wnt signaling in Xenopus. Itoh K, Sokol SY. Genes Dev. 1999 Sep 1;13(17):2328-36. 10.1101/gad.13.17.2328 PubMed 10485853
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