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Addgene

ECD8-pXT7
(Plasmid #32992)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXT7
  • Backbone manufacturer
    R. Freeman/ S. Sokol
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Xenopus RNA expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ecd8
  • Alt name
    Xfz8
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    950
  • Mutation
    Contains the first 230 aa of Xfz8
  • Entrez Gene
    fzd8.L (a.k.a. XELAEV_18030715mg, Xfz8, frizzled8, fz8, fzd8, fzd8-a, fzd8-b)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Digest with Pst1, transcribe with T7 pol for sense RNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ECD8-pXT7 was a gift from Sergei Sokol (Addgene plasmid # 32992 ; http://n2t.net/addgene:32992 ; RRID:Addgene_32992)

  • For your References section:

    Axis determination by inhibition of Wnt signaling in Xenopus. Itoh K, Sokol SY. Genes Dev. 1999 Sep 1;13(17):2328-36. 10.1101/gad.13.17.2328 PubMed 10485853
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