ECD8-pXT7
(Plasmid
#32992)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 32992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepXT7
-
Backbone manufacturerR. Freeman/ S. Sokol
- Backbone size w/o insert (bp) 3000
-
Vector typeXenopus RNA expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameEcd8
-
Alt nameXfz8
-
SpeciesX. laevis (frog)
-
Insert Size (bp)950
-
MutationContains the first 230 aa of Xfz8
-
Entrez Genefzd8.L (a.k.a. XELAEV_18030715mg, Xfz8, frizzled8, fz8, fzd8, fzd8-a, fzd8-b)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site None (unknown if destroyed)
- 3′ cloning site None (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Digest with Pst1, transcribe with T7 pol for sense RNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
ECD8-pXT7 was a gift from Sergei Sokol (Addgene plasmid # 32992 ; http://n2t.net/addgene:32992 ; RRID:Addgene_32992) -
For your References section:
Axis determination by inhibition of Wnt signaling in Xenopus. Itoh K, Sokol SY. Genes Dev. 1999 Sep 1;13(17):2328-36. 10.1101/gad.13.17.2328 PubMed 10485853
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/90/84/95ffa3fc-016d-11e1-9dc1-003048dd6500.pdf.940x940_q85_autocrop.png)